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正文:
穿透式电子
显微镜样品前处理程序
(1) 2.5% 戊二醛 (glutaraldehyde)/0.1M
缓冲溶液 (buffer)(0~4℃)
1~2hr
(2) 0.1M 缓冲溶液 /5% 蔗糖 (0~4℃)
15min
(3) 0.1M 缓冲溶液 /5% 蔗糖 (0~4℃)
15min
(4) 1% 锇 (osmium) /0.1M 缓冲溶液 ( 此步骤以后可在室温下进行 )
1hr
(5) 0.1M 缓冲溶液 /5% 蔗糖 (0~4℃)
15min
(6) 0.1M 缓冲溶液 /5% 蔗糖 (0~4℃)
15min
(7) 50% 酒精
10min
(8) 70% 酒精
10min
(9) 80% 酒精
10min
(10) 90% 酒精
10min
(11) 95% 酒精
10min
(12) 100% 酒精
15min
(13) 100% 酒精
15min
(14) 100% 丙酮
15min
(15) 100% 丙酮
15min
(16) 100% 丙酮 /Spurr's resin 1:1
30-60min
(17) 100% 丙酮 /Spurr's resin 1:3
30-60min
(18) Spurr's resin 1:3
1-3 hr
(19) Spurr's resin 1:3
1-3 hr
(20) 包埋
1-3 hr
(21) 置于 70℃ 烘箱中 8 至 24 小时
出自http://www.bjsgyq.com/
北京显微镜百科