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我读原文书看到光学显微镜的部分时书上介绍很多种

像"bright field microscopy"

"phase contrast microscopy"

"differential interference contrast microscopy"

....等等

但是我刚刚看网路上介绍光学显微镜
只有複式和解剖这两种光学
可以帮我解释上述这几种显微镜吗???
看不太懂原文写什么

北京上光仪器回覆：
bright feild microscopy(亮视野显微镜)就是我们平常使用的显微镜，複式和解剖显微镜都是。phase contrast microscopy(相位差显微镜)利用物体的颜色和吸光性的不同，也就是加强光与暗的对照，使可鑑别显微镜下的观察物，可用于观察活体细胞。 differential interference contrast microscopy，一如相位差显微镜，利用光学特徵的不同，加强因密度而产生光与影的效果。

我想要了解的是这篇文章到底在说些啥?!
萤光显微镜 （的期刊的生物化学 VOL.283、 号 4 页 2454–2464) 后转染，储存格被冲洗磷酸盐缓冲盐和固定在 5 分钟 25 C 后, 跟在 15 分钟跌 C 的甲醇磷酸盐缓冲盐水的 4%甲醛。 固定的储存格被探测兔抗 hNinein 血清 (1:500，我们准备)、 兔抗 Astrin 抗体我们製备 1:500）、 滑鼠抗 Astrin 抗体 (1:500，我们製备) 兔 anti-Aurora 一 (1:500，我们製备)，兔 antipericentrin (1:500，Abcam) 滑鼠抗-BubR1 (1:250，屋宇署生物科学) 滑鼠抗 CENP-E （1:250，Abcam) 滑鼠 anti-tubulin 抗体 （1000 ； Sigma GTU 九八七） 和 （1000 ； 糖尿病 1A，Sigma） 的滑鼠 anti-tubulin 抗体。 DNA 是 4，6-diamidino-2-phenylindole (2 μg/毫升） 染色。 萤光细胞图像被获得一个显微镜 （Olympus) 的 Olympus LSM Fluoview 焦 500 镭射。
Adobe Photoshop 软体 (Adobe Systems） 处理影像。 为了避免潜在的差异，由于褪色的同一个会话过程中收集所有的资料。 图像，然后测量的每个图像的地区和总图元数 circumscribing 主轴轮廓并选择该区域的定量。 图元数获得通过将选定的区域乘以图元强度。
Immunofluorescence Microscopy (THE JOURNAL OF BIOLOGICAL
CHEMISTRY VOL. 283, NO. 4, pp. 2454–2464)
After transfection, the cells were washed with phosphate-buffered saline and fixed with
4% formaldehyde in phosphate-buffered saline for 5 min at 25 °C followed by methanol
at -20 °C for 15 min. The fixed cells were probed with rabbit anti-hNinein serum (1:500,
our preparation), rabbit anti-Astrin antibody (1:500, our preparation), mouse anti-Astrin
antibody (1:500, our preparation), rabbit anti-Aurora A (1:500, our preparation), rabbit
antipericentrin (1:500, Abcam), mouse anti-BubR1 (1:250, BD Biosciences), mouse
anti-CENP-E (1:250, Abcam), mouse anti-tubulin antibody (1:1000; GTU-88, Sigma),
and mouse anti-tubulin antibody (1:1000; DM 1A, Sigma). DNA was stained with
4,6-diamidino-2-phenylindole (2 μg/ml). Immunofluorescent cell images were acquired
using an Olympus LSM Fluoview 500 Confocal laser scanning microscope (Olympus).
Images were processed with Adobe Photoshop software (Adobe Systems). All data were
collected during the same session to avoid potential differences due to fading. Images
were then measured for area and total pixel number for each image by circumscribing
spindle contours and selecting that region for quantitation. Pixel number was obtained by
multiplying the selected area by pixel intensity.